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Image Search Results
Journal: Brain Pathology
Article Title: Neuronal loss without amyloid‐β deposits in the thalamus and hippocampus in the late period after middle cerebral artery occlusion in cynomolgus monkeys
doi: 10.1111/bpa.12764
Figure Lengend Snippet: Double‐immunofluorescence labelling for Aβ with NeuN, GFAP and Iba1 in the ipsilateral thalamus (A) and hippocampus (B) at 12 months after MCAO. Arrows indicate co‐localization of neurons with 4G8. n = 4. Scale bar = 50 μm. Abbreviations: Aβ, β‐amyloid; MCAO, middle cerebral artery occlusion.
Article Snippet: Double‐labeled immunofluorescence For double‐labeled immunofluorescence staining, sections were incubated with mixtures of rabbit and mouse primary antibodies overnight at 4°C: mouse anti‐Aβ 1‐16 (6E10), mouse anti‐Aβ 17‐24 (4G8), mouse anti‐Aβ 40 , mouse anti‐Aβ 42 , rabbit anti‐neuronal nuclei (NeuN; neuronal marker, 1:500, Millipore), rabbit
Techniques: Immunofluorescence